Dental Journal of Iwate Medical University
Online ISSN : 2424-1822
Print ISSN : 0385-1311
ISSN-L : 0385-1311
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Volume 33, Issue 2
Displaying 1-18 of 18 articles from this issue
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  • Effects of elcatonin on matrix calcification of Meckel's cartilage in vitro
    Kiyoto Ishizeki, Tadayoshi Kagiya, Naoki Fujiwara, Hidemistu Harada
    Article type: Article
    2008 Volume 33 Issue 2 Pages 58-69
    Published: August 20, 2008
    Released on J-STAGE: March 08, 2017
    DOIhttps://njjn-jsjc-gov-cn-s-1416.res.gxlib.org.cn:443/rwt/1416/https/MSYXTLUQPJUB/10.20663/iwateshigakukaishi.33.2_58
    JOURNAL FREE ACCESS
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    The effects of elcatonin (ELC) on Meckel's cartilage obtained from 17-day embryonic mice were investigated using cell and organ cultures. Specimens for cell and organ cultures were cultured for 4 weeks in different media as follows: Control medium, alpha-modified Eagle's medium (α-MEM); β-Gly medium, α-MEM plus β-glycerophosphate (β-Gly); ELC medium,α-MEM plus ELC; and β-Gly & ELC medium,α-MEM plus β-Gly plus ELC. Specimens were analyzed by histological and histochemical methods, including immunohistochemistry for alkaline phosphatase (ALPase) activity and bromodeoxyuridine (BrdU) incorporation. Von Kossa staining revealed that the ELC medium inducedeffective calcification similar to β-Gly medium, while BrdU incorporation showed that there was no significant difference in the rates of cell proliferation between Control medium and ELC medium. Histochemical and immunofluorescence staining indicated that ALPase was activated prior to matrix calcification in cell and organ cultures. β-Gly & ELC medium induced synergistically the highest level of matrix calcification and ALPase activity. The present results suggest the possibility that ELC accelerates activity of ALPase in Meckel's chondrocytes and subsequently induces matrix calcification.
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