The Review of High Pressure Science and Technology

High Pressure NMR Enabling a Wide Conformational Search of Proteins

Kazuyuki AKASAKA

2000 Volume 10 Issue 2 Pages 88-94
Published: May 20, 2000
Released on J-STAGE: August 07, 2009

DOIhttps://njjn-jsjc-gov-cn-s-1416.res.gxlib.org.cn:443/rwt/1416/https/MSYXTLUQPJUB/10.4131/jshpreview.10.88

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Many proteins are considered to perform their functions by dynamic excursion to “other” conformations that deviate from the basic structure found in crystal. These “other” conformations have seldom become targets of detailed structural study. The on-line cell high pressure NMR technique developed at Kobe is the only available technique capable of producing “other” conformations of proteins and simultaneously reporting their structures at residue-specific resolution using multi-dimensional NMR spectroscopy. The principle is based on the recognition that the partial molar volume of a protein strongly depends on its conformational state. Examples are given from two proteins, basic pancreatic trypsin inhibitor and the Ras-binding domain of RalGEF.

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Recent Advances in High-Pressure Infrared Spectroscopic Studies on Proteins

Wojciech DZWOLAK, Minoru KATO, Yoshihiro TANIGUCHI

2000 Volume 10 Issue 2 Pages 95-100
Published: May 20, 2000
Released on J-STAGE: August 07, 2009

DOIhttps://njjn-jsjc-gov-cn-s-1416.res.gxlib.org.cn:443/rwt/1416/https/MSYXTLUQPJUB/10.4131/jshpreview.10.95

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This paper aims to review recent trends and developments in high-pressure FTIR studies on proteins. Methodological principles of research in this field, the assignment of the diagnostically-useful IR bands, as well as several examples illustrating the application of IR spectroscopy in high pressure studies on proteins are all within the scope of this article. This work is also an attempt to show that IR spectroscopy can find application not only for studies of the pressure-induced unfolding of protein structure, but also for the examination of minor unfolding events or local conformational changes induced by high pressure. A more elaborate discussion of the high-pressure study on bovine α-lactalbumin is also presented.

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Compressibility-Structure Relationship of Proteins

Kunihiko GEKKO

2000 Volume 10 Issue 2 Pages 101-107
Published: May 20, 2000
Released on J-STAGE: August 07, 2009

DOIhttps://njjn-jsjc-gov-cn-s-1416.res.gxlib.org.cn:443/rwt/1416/https/MSYXTLUQPJUB/10.4131/jshpreview.10.101

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Protein dynamics is a basis for understanding the principles of constructing three-dimensional structures and the structure-function relationship of proteins. A novel measure of the flexibility of protein in water is compressibility because it is directly linked to the volume fluctuation. It is known that the adiabatic compressibility sensitively reflects the structural characteristics of proteins although it is a thermodynamic quantity. This article is a review of recent studies on the compressibility-structure relationship of native globular proteins.

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Enzyme Reaction under High Pressure

Shoichi MAKIMOTO

2000 Volume 10 Issue 2 Pages 108-115
Published: May 20, 2000
Released on J-STAGE: August 07, 2009

DOIhttps://njjn-jsjc-gov-cn-s-1416.res.gxlib.org.cn:443/rwt/1416/https/MSYXTLUQPJUB/10.4131/jshpreview.10.108

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From the many studies in enzyme reactions under high pressure, the activation volume for the catalytic process and the reaction volume for the dissociation process of the Michaelis complex evaluated from the pressure dependence of the enzyme reaction are found to be powerful tools for studying the reaction mechanisms in these processes. The above volume changes not only clarify the enzyme reaction mechanism, but also may explain the relationship between the high catalytic efficiency/specificity and the reactaonmechanism. As an example of an enzyme reaction, the reaction mechanism of α-chymotrypsin (α-CHT) catalysis is discussed in terms of the volume change.

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Control of Products for the Enzyme Reaction by High Hydrostatic Pressure

Tadashi MATSUMOTO, Shoichi MAKIMOTO, Yoshihiro TANIGUCHI

2000 Volume 10 Issue 2 Pages 116-123
Published: May 20, 2000
Released on J-STAGE: August 07, 2009

DOIhttps://njjn-jsjc-gov-cn-s-1416.res.gxlib.org.cn:443/rwt/1416/https/MSYXTLUQPJUB/10.4131/jshpreview.10.116

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To investigate the possibility that pressure can control the products for the enzyme reactions, pressure effects on the time course of the products' composition accompanying the hydrolysis of maltooligosaccharides [maltotetraose (G4), maltopentaose (G5), and maltohexaose (G6) ] and amylose catalyzed by porcine pancreatic α-amylase (PPA) were measured up to 300 MPa at 30°C. The composition of products for the hydrolysis of G5 substrates changed slightly by compression. But for G4, G6 and amylose substrate, pressure induced some changes in the composition of the products. These results tell us that pressure is one of the efficacious tools to control the products of enzyme reaction of α-amylase. The mechanism of an interesting pressure-induced reaction catalyzed by PPA is discussed in terms of the volume differences among enzyme-substrate (ES) complexes.

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Water in the Earth's Interior

Is the mantle transition zone a water reservoir?

Toru INOUE

2000 Volume 10 Issue 2 Pages 124-133
Published: May 20, 2000
Released on J-STAGE: February 05, 2010

DOIhttps://njjn-jsjc-gov-cn-s-1416.res.gxlib.org.cn:443/rwt/1416/https/MSYXTLUQPJUB/10.4131/jshpreview.10.124

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H₂O is an important volatile material in the Earth, and it affects the physical properties (e. g. density, elastic velocity, viscosity, rheobgical property, diffusion, electrical conductivity and melting temperature) of the Earth's materials. Recently, it has been darified that significant amounts of H₂O can be accommodated in β and γ phases of olivine, which means that the mantle transition zone has the potential of being a water reservoir in the Earth. In this paper, I review the H₂O contents, lattice parameters and elastic properties of hydrous β and γ phases, the effect of H₂O on the phase transformation of olivine, and the possibilities of water transportation into the mantle transition zone are discussed on the basis of these experimental data.

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First-Principles Investigation of Unknown Structures and Properties of Materials under Pressure

Yoshitaka TATEYAMA

2000 Volume 10 Issue 2 Pages 134-141
Published: May 20, 2000
Released on J-STAGE: August 07, 2009

DOIhttps://njjn-jsjc-gov-cn-s-1416.res.gxlib.org.cn:443/rwt/1416/https/MSYXTLUQPJUB/10.4131/jshpreview.10.134

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The constant-pressure first-principles molecular dynamics (CP-FPMD) method shows its great power in the investigation of high-pressure phenomena which are difficult to observe in experiments. In this article, I present a survey of this CP-FPMD method as well as a new technique we developed for the determination of transition states. I also illustrate their ability with our investigations of high-pressure synthesis of BCN heterodiamonds and pressure induced metallization of organic monomolecular crystals.

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Science and Technology in the 21st Century

Muneyuki DATE

2000 Volume 10 Issue 2 Pages 142-147
Published: May 20, 2000
Released on J-STAGE: August 07, 2009

DOIhttps://njjn-jsjc-gov-cn-s-1416.res.gxlib.org.cn:443/rwt/1416/https/MSYXTLUQPJUB/10.4131/jshpreview.10.142

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Ten Years of the Japan Society of High Pressure Science and Technology

Yasuhiko SYONO

2000 Volume 10 Issue 2 Pages 148-152
Published: May 20, 2000
Released on J-STAGE: August 07, 2009

DOIhttps://njjn-jsjc-gov-cn-s-1416.res.gxlib.org.cn:443/rwt/1416/https/MSYXTLUQPJUB/10.4131/jshpreview.10.148

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Round-table Talk

Past, Present, and Future of the JSHP

[in Japanese]

2000 Volume 10 Issue 2 Pages 153-166
Published: May 20, 2000
Released on J-STAGE: August 07, 2009

DOIhttps://njjn-jsjc-gov-cn-s-1416.res.gxlib.org.cn:443/rwt/1416/https/MSYXTLUQPJUB/10.4131/jshpreview.10.153

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Tracing the Foundation of the JSHP (Minutes of the Meeting)

Hironobu Kubota

2000 Volume 10 Issue 2 Pages 167-168
Published: May 20, 2000
Released on J-STAGE: August 07, 2009

DOIhttps://njjn-jsjc-gov-cn-s-1416.res.gxlib.org.cn:443/rwt/1416/https/MSYXTLUQPJUB/10.4131/jshpreview.10.167

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