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Volume 15 (1974)
- Issue 6 Pages 411-
- Issue 5 Pages 331-
- Issue 4 Pages 219-
- Issue 3 Pages 135-
- Issue 2 Pages 85-
- Issue 1 Pages 1-
Volume 15, Issue 3
Displaying 1-10 of 10 articles from this issue
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Hideaki MIYATA1974 Volume 15 Issue 3 Pages 135-146
Published: June 05, 1974
Released on J-STAGE: December 11, 2009
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Akio YAMADA1974 Volume 15 Issue 3 Pages 147-152_1
Published: June 05, 1974
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe acute toxicities of di-n-butyl phthalate (DBP) and di-(2-ethyl hexyl) phthalate (DOP) were studied in orally administered mice, and the subacute toxicities of the chemicals in rats by oral administration for 3 weeks. Further study of hepatotoxicity of DOP was performed by oral administration for 3-weeks in rats.
LD50 of DBP and DOP to mice in 168 hours were 15.9 and 49.7ml/kg, respectively.
In the studies of subacute toxicities of DBP and DOP for 3 weeks in rats, it was observed that mortality was increased and body weight gain was decreased with increasing doses. Especially liver enlargement was observed by 3-week administration of DBP (1 or 5ml/kg/day) or DOP (4 or 10ml/kg/day) for 3 weeks.
Liver constituents, serum enzymatic activities and BSP clearance were measured in relation to the liver enlargement after oral administration of 5ml/kg/day of DOP for 3-weeks. Water-, protein- and fat- contents in the liver and activities of ALP, LDH, GOT and GPT in the serum showed no differences between control and test groups in spite of liver enlargement, but it was observed that glycogen content in the liver was decreased and the BSP clearance was inhibited.
The inhibited BSP clearance by DOP administration was recovered a week after discontinuation of administration, whereas liver enlargement recovery was observed 2 weeks after the discontinuation.View full abstractDownload PDF (840K) -
Analytical Method of Chlorobenzilate Residue in Agricultural ProductsKumiko OHTUKI, Mitsuharu TAKEDA, Hiroya TANABE1974 Volume 15 Issue 3 Pages 153-158_1
Published: June 05, 1974
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSChlorobenzilate was investigated to establish the analytical method of it by ECD-gas chromatography.
From activated carbon column, organochlorine insecticides including chlorobenzilate were eluted by the mixture of n-hexane and ethylether (1:4), but δ-BHC was not recovered in the step.
In florisil column chromatography, chlorobenzilate was completely separated from other organochlorine insecticides, that is, the latter were eluted by the mixture of n-hexane and ethylether (4:1) and the former was contained in the second eluate of n-hexane, acetone and ethylether (3:1:1).
In the analytical method of organochlorine insecticide residues including chlorobenzilate in some fruits devised in this study, recovery test of them added to samples gave good results (70% of aldrin in rind of summer orange to 109% of pp′-DDT in summer orange).View full abstractDownload PDF (670K) -
Akira SUZUKI, Tsutomu KAWANISUI, Hirotaka KONUMA, Sumie TAKAYAMA1974 Volume 15 Issue 3 Pages 159-176_1
Published: June 05, 1974
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSIsolation and identification of Salmonella types were carried out on 1903 lots, or 6523 samples, of poultry meat imported into Japan over a 15-month period from July, 1971, to October, 1972. The following results were obtained.
1) The rate of Salmonella contamination of imported poultry meat varied with the exporting country and the kind of domestic fowls. The average rate of such contamination was 10.3 and 24.2% as determined by samples and lots, respectively.
2) The rate varied greatly with the exporting country. It was close to the average positive rate of samples for the poultry meat imported from the United States (10.7%) and China (9.3%). It was rather higher for that imported from New Zealand (44.4%), the Netherlands (28.5%), France (26.7%), and Canada (17.6%), whereas lower for that imported from Denmark (3.8%).
3) When determined by the kind of poultry, the average rate of contamination in samples was 10.3% for chicken and 10.6% for meat of the other poultry than the chicken.
4) When determined by the portion of chicken, the rate of contamination was about 60%, or the highest, in the “communited” portion.
5) Isolated organisms were classified into O antigen groups and sero types. A total of 32 types were detected and classified into the C1 group (42.8%), B group (29.0%), and E group (15.8). The first 10 types of them are listed in the decreasing order of frequency as follows: S. infantis, S. anatum, S. typhimurium, S. potsdam, S. thompson, S. heidelberg, S. saint-paul, S. kottbus, S. enteritidis, and S. derby. This pattern of Salmonella types was clealy different from that of horse or kangaroo meat.
6) The pattern of Salmonella types detected from poultry meat imported from the United States and some European countries was found to be nearly similar, while there was a marked difference in that from China.View full abstractDownload PDF (1858K) -
Shihoko TERADA, Shoji KIMURA1974 Volume 15 Issue 3 Pages 177-180_1
Published: June 05, 1974
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA simple method for the determination of the preservatives (dehydroacetic acid, benzoic acid, butyl p-hydroxybenzoate) in foods was studied. The food preservatives were isolated from food by steam distillation, extracted by ethylether and separated by thin layer chromatography. Each spot on the thin layer chromatogram was scanned by a dual wave length densitometer under ultraviolet ray. The recoveries of preservatives in foods were 85-102%.View full abstractDownload PDF (487K) -
Fumio MIYAZAWA, Kuniko INADA, Reiko SANO, Tatuo NAKANO1974 Volume 15 Issue 3 Pages 181-187_1
Published: June 05, 1974
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA new selective medium, rose bengale lactose agar (RBL agar), for the detection of contaminated coliform organisms in foods was demonstrated.
In our previous papers, it was reported that xanthene dyes having halogen atoms in their molecules show photodynamic lethal activities against bacteria, especially grampositive bacteria. Expecting this action of dye, we added rose bengale as a photosensitizer at a concentration of 20μg/ml to the medium, and also added lactose (1%) and bromothymol blue (40μg/ml) as an indicator to detect lactose-fermenting organisms. It was observed that most of the test organisms belonging to Micrococcaceae or Bacillus were inhibited to grow on RBL agar, if they were irradiated with visible light, while gram-negative bacteria, especially those of Enterobacteriaceae, grew well. Colonies of lactose-nonfermenting organisms were found to be medium colored, whereas coliform organisms had either orange-red colonies with or without colorless periphery.
The isolation of coliform organisms from several types of foods, such as soy beancurd, juice and frozen food, was performed using this medium. It was found that, so far as the orange-red colored colonies were picked up they were all classified into coliform organism.View full abstractDownload PDF (2581K) -
Yataro KOKUBO1974 Volume 15 Issue 3 Pages 188-194_1
Published: June 05, 1974
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSGrowth temperature characteristics of 10 strains of psychrotrophic bacteria, which consisted of each 2 strains of Pseudomonas sp. (fluorescent type), Pseudomonas sp. (nonfluorescent type), Flavobacterium sp., Coryneforms sp. and Micrococcus sp., isolated from frozen pork were studied, since these 5 strains had been the most predominant contaminants of the frozen meat. Growth curves obtained on the 10 strains at varying temperatures covering 0 to 50°C with 5°C intervals were compared with those of 8 reference strains of stock cultures, Pseudomonas fluorescens, Pseudomonas fragi, Flavobacterium suaveolens, Cytophaga (Guppy B), Escherichia coli, Microbacterium thermosphactum, Micrococcus caseolyticus and Staphylococcus aureus. A temperature gradient incubator model TN-3 of Toyo Kagaku Sangyo Co. was used in this experiment.
Regardless of the genera of the organisms minimum temperatures for the growth of all the isolates tested were below 0°C and the optimum ones were 25 or 30°C. The minimum generation time of the isolates varied from 29 to 65min. Among these isolates, both Pseudomonas fluorescent types and non-fluorescent ones showed maximum growth temperatures of 35 and 40°C respectively, while those of the other organisms were 30°C. The growth rates of the psychrotrophic stock strains at 0°C were a little lower than those of the Pseudomonas sp. of the isolates.View full abstractDownload PDF (949K) -
Kuniyuki TANAKA, Ryuji AMANO, Kohei KAWADA, Hiroya TANABE1974 Volume 15 Issue 3 Pages 195-200_1
Published: June 05, 1974
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSIn this paper, a gas liquid chromatography for detection and determination of trichothecenes which were toxic metabolites of Fusarium sp. was described.
Trimethyl silylation, acethylation and trifluoroacethylation of trichothecenes were studied. From these results, it was found that trimethylsilyl ether was more suitable than acetate or trifluoroacetate for gas liquid chromatography. Trimethyl silylation of nivalenol and the related compounds (fusarenon and diacethylnivalenol) was difficult. They were imperfectly silylated by bis-trimethylsilyl acetamide, hexamethyl disilazane+trimethyl chlorosilane or N-trimethylsilyl imidazole. However using a mixture of N-trimethylsilyl imidazole, trimethyl chlorosilane and pyridine as silylating reagent they were easily and completely converted to trimethylsilyl ethers.
SE-30 as well as OV-17 was the most suitable liquid phase for detection and determination for trimethylsilyl ethers of trichothecenes by gas liquid chromatography, but another phases such as QF-1, XE-60 and NPGA were also useful.View full abstractDownload PDF (680K) -
Kinetical Studies on the Nitrosation of Piperidine and MorpholineTakashi YAMADA, Miyako YAMAMOTO, Akio TANIMURA1974 Volume 15 Issue 3 Pages 201-205_1
Published: June 05, 1974
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSRates of formation of nitrosopiperidine (NP) and nitrosomorpholine (NM) were studied.
Initial rates of formation of NP from piperidine and sodium nitrite were calculated from the absorbance at 260nm after decomposition of nitrite with sulfamic acid. They were proportional to [piperidine] [NaNO2]2 both at pH 4.2 and 3.0. Initial rates of formation of NM from morpholine and sodium nitrite at pH 4.2 and 3.0 were also proportional to [morpholine] [NaNO2]2.
The effect of SCN- on the formation of NP at various pH values was also studied. SCN- in concentration of 15mM accelerated the initial rate of formation of NP by three times compared with one in the absence of SCN- at pH 1.25, but accelerated only by 1.2 times at pH 3.5. According to Boyland et al., the content of SCN- in human saliva was estimated to be 2-5.7mM. Therefore, SCN- in human saliva does not seem so much effective to the rate of formation of NP.View full abstractDownload PDF (639K) -
Nitrite and Nitrate Contained in Human SalivaMoto-o HARADA, Hajimu ISHIWATA, Yoko NAKAMURA, Akio TANIMURA, Morizo I ...1974 Volume 15 Issue 3 Pages 206-207
Published: June 05, 1974
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDownload PDF (272K)
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