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Volume 18 (1977)
- Issue 6 Pages 487-
- Issue 5 Pages 405-
- Issue 4 Pages 309-
- Issue 3 Pages 217-
- Issue 2 Pages 131-
- Issue 1 Pages 1-
Volume 18, Issue 3
Displaying 1-12 of 12 articles from this issue
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Parp. 2 Application of Detergent Containing Sugar Fatty Acid Ester and Potassium Pyrophosphate to Various Fruits and VegetablesZen-ichi MORI, Jun-ichi TAMURA1977 Volume 18 Issue 3 Pages 217-224_1
Published: June 05, 1977
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSRemoval effects of residual pesticides from cabbage, welsh onion, spinach, salted cucumber, grape, apple, white peach and satuma mandarin by washing with detergent containing sugar fatty acid ester and potassium pyrophosphate (detergent A), liquid detergent for kitchen use (LD) and water only (control) were studied. Residual inorganic pesticides such as Bordeaux, arsenic and lead were removed most efficiently by detergent A. As to residual organic pesticides such as organophosphorus insecticides, the effect of LD washing was found to be somewhat higher than that of detergent A washing, but the residues were scarecely removed by water washing.View full abstractDownload PDF (980K) -
Shigenobu WATANABE, Michiko NUKIYAMA, Fumiko TAKAGI, Katsuhiko IIDA, T ...1977 Volume 18 Issue 3 Pages 225-230_1
Published: June 05, 1977
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSIn USA, contamination of PBBs, which are used to increase the heat stability of plastics were reported. Following the incident, maximum allowable limits of PBBs for meat, milk fat, eggs and feed were set by FDA in 1974.
From those, an analytical method concerning PBBs contaminations of food and plastics, was discussed. It was cleared that the determination for PCBs was successfully adapted for PBBs analysis, then contamination of fishes, dairy products and plastics were investigated. All samples were found to be free from PBBs contaminations.View full abstractDownload PDF (538K) -
Kumiko SASAKI, Mitsuharu TAKEDA, Mitsuru UCHIYAMA1977 Volume 18 Issue 3 Pages 231-237_1
Published: June 05, 1977
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA simple method for the fluorometric assay of oxytetracycline (OTC) residue in meats and fishes was presented.
A homogenized sample was extracted with 1N hydrochloric acid containing β-mercato-propionic acid (β-MPA). The extract was cleaned up by Amberlite XAD-8 column chromatography. The column was washed with water and methanol, and then eluted with 0.005N sodium hydroxide solution after removed completely bubbles on the column with ascending stream of water. The eluate was collected in a flask containing 1N hydrochloric acid and β-MPA, and concentrated just to dryness at 35°C. The residue obtained was dessolved in 4N hydrochloric acid containing β-MPA, and converted to apo-OTC by heating. The solution was adjusted to 5ml with sodium borate buffer after neutrized with 6N sodium hydroxide solution.
The excitation spectrum was measured over the 340-410nm at the emission wavelength of 447nm and excitation intensity was obtained from the following equation, Ex=Ex390-Ex350.
The excitation intensity was employed for the fluorometric determination, because the eluate of the samples from the Amberlite XAD-8 column contained the materials which gave a similar pattern to OTC in the emission intensity.
The efficiency of the method described was evaluated by the samples fortified with OTC (0.25ppm level), and average recoveries ranged from 33 to 37%.View full abstractDownload PDF (850K) -
Effects of Xanthene Dyes on the Activities of Trypsin and ChymotrypsinKozo KIDO, Tadao WATANABE1977 Volume 18 Issue 3 Pages 238-243_1
Published: June 05, 1977
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSEffects of xanthene dyes on the esterase activities of trypsin and chymotrypsin were examined by using synthetic substrates, Nα-benzoyl-L-arginine ethyl ester and Nα-benzoyl-L-tyrosine ethyl ester, respectively. Xanthene dyes strongly inhibited the tryptic and chymotrypsic activities in proportion to the increasing of light intensity.
Also, the concentration and time for 50% inhibition by xanthene dyes and treating period were examined. From these results, it was found that the extent of inhibition was in the following order: rose bengale>phloxine>erythrosine>eosine. Km and kcat values were calculated, using Lineweaver-Burk's plot. The results showed that Km values from both enzymes treated with xanthene dyes were smaller as compared with those of treated enzymes, and kcat values were larger. It was assumed that active sites in the molecules of trypsin and chymotrypsin were degraded by the photo-effect or masked with the dyes.View full abstractDownload PDF (679K) -
Takenori MARUYAMA, Toshiaki USHIGUSA, Hiromu KANEMATSU, Isao NIIYA, Ma ...1977 Volume 18 Issue 3 Pages 244-251_1
Published: June 05, 1977
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSAnalysis of phthalate esters by a partly modified essential oil distillator was examined, and approximately satisfactory recovery was obtained with water, non-fatty food and fatty food by taking samples containing about 2g of organic substance (protein and carbohydrate). Over 99% of organochlorine pesticides and PCB, which show an interfering peak in the gas chromatogram, were removed by the combination of zinc powder addition, refluxing and Florisil batch method. In the final procedure of this pretreatment, a known quantity of pyrene was added, and since determination was made by the internal standard method, precision of the determination was improved. Further, the number of reagents and apparatus used were so small that the blank values became lower.View full abstractDownload PDF (936K) -
Akio NAKAMURA, Takashi KASHIMOTO1977 Volume 18 Issue 3 Pages 252-259_1
Published: June 05, 1977
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSAuthors selected sulfur compounds usually contained in oil as indicator for the study of oil pollution in marine products.
It was found by FPD-GC analysis that marine products, especially fresh oyster contains similar type of sulfur compounds as found in crude oil. Sulfur compounds contained both in crude oil and oyster were isolated by the combination of two different chromatography and identified as dibenzothiophene derivatives.
Thus organic sulfur compounds are considered to be a good parameter for oil pollution in marine products.
This finding suggestes that these contamination of oil components in oyster are due to oil spill or dump out.
As an another method for studying oil pollution or spill, high pressure liquid chromatography (HPLC) technique appears to be also applicable for the comparison of heterocyclic aromatic compounds such as dibenzothiophene derivatives isolated from different sources.View full abstractDownload PDF (802K) -
Hideaki MIYATA, Takashi KASHIMOTO, Nobuharu KUNITA1977 Volume 18 Issue 3 Pages 260-265_1
Published: June 05, 1977
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSBecause of their chemical stability and their lipophilic nature, it is possible that polychlorodibenzofurans (PCDFs) released into the environment could accumulate in human tissue via food sources. Omentums, livers and a mamma of normal human were used for the PCDFs analysis. PCDFs were detected and determined by means of GC-MS fragmentography. Pentachlorodibenzofuran was detected in three out of five omentums, one of four livers and in a mamma sample at the detection limit of 5ppt. Their levels ranged from n. d. to 45.4ppt. Tetra- and hexa-chlorodibenzofuran were not detected.
At the same time, analysis of PCB and PCDF was made in seven samples of Kanemi rice oil which caused “Kanemi Yusho.” The concentration of PCB in the oil were 0.9 to 1, 029ppm and the concentration of PCDF were 0.0021 to 5.4ppm, respectively. The ratios of PCDF vs. PCB concentration in Kanemi rice oils were extraordinary high in comparison with that of commercially available PCB preparation containing 40% chlorine (KC-400). In view of its strong toxicity, it seems very likely that PCDF is a compound which caused “Kanemi Yusho” together with PCB.View full abstractDownload PDF (729K) -
Hironoshin KAWASAKI, Seizaburo KANOH1977 Volume 18 Issue 3 Pages 266-272_1
Published: June 05, 1977
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSWe investigated on the application of Limulus coagulation test (L-test) which has been used for the detection of endotoxin to the hygienic bacteriological field and obtained the following results.
1) It was found that the inhibitors of L-test in some kind of foods could be removed by chloroform treatment.
2) Results of L-test were not paralleled to total bacterial counts included gram positive bacteria but an excellent correlation existed between both detection of endotoxin by L-test and gram negative bacteria by bacterial cultivation in foods and water.
3) The test could be also applied to the detection of endotoxin in water or milk which could not be detected by bacterial cultivation.
From these data, it was considered that L-test could be applied to rapid detection of gram negative bacteria or the past contamination by gram negative bacteria in the hygienic bacteriological field.View full abstractDownload PDF (888K) -
Yasuhiro YAMATO, Manabu SUZUKI, Tadao WATANABE1977 Volume 18 Issue 3 Pages 273-277_1
Published: June 05, 1977
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSAnalysis of sulfur containing organophosphorus insecticides by gas chromatograph with a dual channel flame photometric detector (FPD-GLC) and data processings using a digital integrator were investigated.
With P mode, the calibration curves of all insecticides employed showed the linear range from zero to hundreds or thousands nanograms. With S mode, also, similar results were obtained, however, these indicated the linearity with only log-log scale. Moreover, with the dual channel FPD-GLC attached with digital integrator, simultaneous identifications of the insecticides contained sulfur and phosphorus in the molecule could be done.
The resolution of salithion, malathion, fenitrothion, ethion, methyl trithion, and EPN were obtained on Apolar 10C. Ronnel, fenitrothion, ethion, and EPN were separated on QF-1. From above results, Apolar 10C was useful for organophosphorus insecticides analysis.
Many interfering peaks were shown at the shorter retention time on the gas chromatograms of vegetable extracts in the S mode. But, few interfering peaks were shown in the P mode.View full abstractDownload PDF (608K) -
Masahiko UETA, Masanori MAZAKI1977 Volume 18 Issue 3 Pages 278-282_1
Published: June 05, 1977
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSAn anion exchange high speed liquid chromatographic method was presented for the simultanious separation and determination of sorbic acid, benzoic acid and saccharin in several foods. Liquid foods such as orange juice and fermented milk were injected into the chromatograph after filtration through a 0.45μm pore menbrane filter. Solid foods such as cooked bean and fish jelly products (“Kamaboko”) were extracted with borate buffer solution (pH 9.2) and filtered through menbrane filter prior to injection. Samples were eluted from a Permaphase AAX column with 10-3M sodium perchloride solution in pH 8, 10-5M borate buffer solution and were determined with a UV detector. Average recoveries of sorbic acid, benzoic acid and saccharin from the samples were 90.1 to 106.5%. However, this method have a problem that samples containing sodium chloride may result the false determination.View full abstractDownload PDF (531K) -
Takenori MARUYAMA, Chiaki MURAKAMI, Hiromu KANEMATSU, Isao NIIYA, Masa ...1977 Volume 18 Issue 3 Pages 283-289
Published: June 05, 1977
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDownload PDF (657K) -
Hideyo SUZUKI, Masatake TOYODA, Yoshio ITO, Masahiro IWAIDA, Hiroko NO ...1977 Volume 18 Issue 3 Pages 290-295
Published: June 05, 1977
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDownload PDF (733K)
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