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Volume 41 (2000)
- Issue 6 Pages 347-
- Issue 5 Pages 297-
- Issue 4 Pages 233-
- Issue 3 Pages 163-
- Issue 2 Pages 95-
- Issue 1 Pages 1-
Volume 41, Issue 6
Displaying 1-10 of 10 articles from this issue
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Originals
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Naoko OZEKI, Eiji UENO, Yuko ITO, Hisao OKA, Tomoko HAYASHI, Yuko ITAK ...2000 Volume 41 Issue 6 Pages 347-352
Published: December 25, 2000
Released on J-STAGE: January 11, 2008
JOURNAL FREE ACCESSWe established an analysis method of food colors, turmeric oleoresin, gardenia yellow, and annatto extract (including annato, water-soluble), in foods using reversed-phase thin-layer chromatography/scanning densitometry. The method involves the following three steps: 1) clean-up of the colors with a C18 cartridge, 2) separation of the colors by reversed-phase C18-TLC using acetonitrile-tetrahydrofuran-0.1 mol/L oxalic acid (7 : 8 : 7) as a solvent system and 3) measurement of visible absorption spectra of the colors using scanning densitometry without isolation of the colors. In order to investigate the capability of the present method, 89 commercial foods were analyzed, and their chromatographic behavior and spectra were observed. The separation and the spectra obtained were not affected by coexisting substances in the foods. The spots always gave the same Rf values and spectra as the standards with good reproducibility. The present method is considered to be useful for the rapid analysis of turmeric oleoresin, gardenia yellow, and annatto extract (including annatto, water-soluble) in foods.View full abstractDownload PDF (1560K) -
Jun SUZUKI, Rie MURATA, Satoshi MOROZUMI, Iwao MURATA, Hiroyuki TSUKUD ...2000 Volume 41 Issue 6 Pages 353-356
Published: December 25, 2000
Released on J-STAGE: January 11, 2008
JOURNAL FREE ACCESSMetagonimiasis is caused by metacercariae of Metagonimus yokogawai, which is mainly found in raw fresh-water fish such as ayu (Plecoglossus altivelis), ugui (Tribolodon hakoensis) and shirauo (Salangichthys microdon). Human stool examination has shown an increase in metagonimiasis since 1990 in Tokyo.
Since shirauo is more popular as a raw fish than ayu and ugui, we investigated M. yukogawai metacercariae in shirauo retailed in Tokyo from 1998 to 1999.
In our study, microscopic examination revealed that metacercariae could be frequently observed in the muscle tissues, and the fins and tail of shirauo taken from Lake Kasumigaura, Ibaraki Prefecture. The rate of infection of metacercariae in these fish was 100% in August 1999 and the maximum infection was 314 metacercariae per fish in September 1999. In contrast, the rate of infection in shirauo from other districts ranged from 0 to 16%.
These results suggest that the foodborne infection of metagonimiasis occurring in Tokyo may have been partly due to the high level of M. yokogawai metacercariae in shirauo from Lake Kasumigaura.View full abstractDownload PDF (354K)
Notes
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Sumiko TSUJI, Ikuko MATSUMURA, Yumiko NAKAMURA, Yasuhide TONOGAI2000 Volume 41 Issue 6 Pages 357-363
Published: December 25, 2000
Released on J-STAGE: January 11, 2008
JOURNAL FREE ACCESSSeparation and determination of subsidiary colors, raw materials and intermediates (organic impurities) in commercial Food Yellow No. 5 (Y-5, sunset yellow FCF) were studied under various HPLC conditions. Separation of the trisodium salt of 7-hydroxy-8-[(4-sulfophenyl)azo-1,3-naphthalenedisulfonic acid (SA-G) and the disodium salt of 4,4'-(diazoamino)dibenzenesulfonic acid (DAADBS) was established by HPLC using L-column ODS and a gradient system of 0.02 mol/L ammonium acetate solution (after isocratic elution for 10 min as a first step) and a mixture of acetonitrile and water (7 : 3). The organic impurities in 39 samples of Y-5 submitted for the inspection in fiscal year 1998 were determined using the proposed HPLC conditions. It was found that the total amount of subsidiary colors in the samples was less than 1/5 of the permitted limit, and the total amount of raw materials and intermediates was not more than half of the limit.View full abstractDownload PDF (673K) -
Nobuyuki TAKEDA, Hiroki NISHIUMI2000 Volume 41 Issue 6 Pages 364-367
Published: December 25, 2000
Released on J-STAGE: January 11, 2008
JOURNAL FREE ACCESSA rapid and reliable quantitative screening procedure was developed for analysis of oxytetracycline (OTC) in animal-derived foods. The procedure involves clean-up using metal chelate (MC) resin and on-line confirmation. Samples were homogenized in 0.25% metaphosphoric acid-methanol-acetonitrile (6 : 2 : 2). OTC was selectively extracted onto MC resin preloaded with Cu2+ and determined by HPLC with fluorescence detection using a Mg2+-containing mobile phase.
Recoveries at levels of 0.1 and 1 μg/g were 80.8∼86.6% (average : 83.8%) with the coefficient of variation of 0.7∼6.0%. The limit of quantitation was set at 0.02 μg/g. Forty samples of meat and fish were analyzed and OTC was found in unagi kabayaki (0.04 μg/g) and in shrimp (0.39 μg/g).
The presence of OTC in the samples was confirmed by the on-line confirmatory technique, that gives visual evidence of whether the equivalent peak is OTC or not, based on its disappearance on the chromatogram when a Mg2+-free mobile phase is used.View full abstractDownload PDF (379K) -
Tadashi SAKAI, Kazuhiro SUGAMOTO, Nozomu ETO2000 Volume 41 Issue 6 Pages 368-370
Published: December 25, 2000
Released on J-STAGE: January 11, 2008
JOURNAL FREE ACCESSCytotoxicity of 4-hydroxy-2E-hexenal (HHE), a hepatotoxic aldehyde, to Chinese hamster lung fibroblast V79 and Balb/c mouse semi-normal cell line BALB/3T3 clone A31 cells was examined. The 50%-inhibitory concentrations to V79 and A31 were 3 μmol/L and 16 μmol/L, respectively. Judging from the sensitivity, HHE might show cytotoxicity below these concentrations. Changes in HHE and malonaldehyde (MA) contents were also examined in yellowtail meat stored at -20°C. HHE content increased up to 60.82±4.39 μmol/kg meat during 28 weeks of storage. In contrast to HHE, MA content increased only slightly in the meat during 28 weeks of storage. From the viewpoint of food hygiene, it is noteworthy that the HHE content increased even when meat was stored at -20°C.View full abstractDownload PDF (362K) -
Masako ITO, Toshihiro NAGAYAMA, Ichiro TAKANO, Maki KOBAYASHI, Yasuhir ...2000 Volume 41 Issue 6 Pages 371-376
Published: December 25, 2000
Released on J-STAGE: January 11, 2008
JOURNAL FREE ACCESSSimplified determination of cyclosulfamuron in agricultural products by HPLC with a UV detector was investigated.
The homogenate of agricultural products was extracted with acetone. The crude extract was partitioned between 10% sodium chloride solution and ethyl acetate-n-hexane (1 : 1) solution, and the ethyl acetate-n-hexane layer was evaporated to dryness. The residue was dissolved in n-hexane, and after the solution had been refined with ACCUCAT™, the methanol solution was analyzed by liquid chromatography. Cyclosulfamuron was detected in terms of the absorbance of 260 nm.
Recoveries at the 0.1 ppm level were more than 73% from agricultural products. Detection limits were 0.01 ppm in samples.View full abstractDownload PDF (483K) -
Satoru NEMOTO, Satoshi TAKATSUKI, Kumiko SASAKI, Masatake TOYODA2000 Volume 41 Issue 6 Pages 377-380
Published: December 25, 2000
Released on J-STAGE: January 11, 2008
JOURNAL FREE ACCESSFish purchased from 6 stores were assayed for nonylphenol (NP). NP was detected in 24 of 35 samples at the level of 9∼800 ng/g (LOD=8 ng/g). NP levels in fish varied depending on the store where the fish were purchased.
Migration of NP into n-heptane from wrapping films and polystyrene trays used to pack the fish samples was investigated. Migration levels of NP from wrapping film used to pack fish in which NP was detected were 70∼931 ng/cm2 and those from wrapping film used to pack fish in which NP was almost never detected were nd∼21 ng/cm2. NP was migrated from high-impact polystyrene trays at the level of 120∼270 ng/cm2 but did not migrate from polystyrene foam trays. The migration of NP from tray to fish may have been impeded by the presence of a water-absorbing sheet between fish and tray. These results suggested that most of the NP detected in fish was due to migration from wrapping film.View full abstractDownload PDF (361K) -
Satoshi TAKATSUKI, Satoru NEMOTO, Tomoaki TSUTSUMI, Kumiko SASAKI, Mas ...2000 Volume 41 Issue 6 Pages 381-386
Published: December 25, 2000
Released on J-STAGE: January 11, 2008
JOURNAL FREE ACCESSA method was developed for analysis of acibenzolar-S-methyl (benzo[1,2,3]thiodiazole-7-carbothioic acid S-methyl ester, BTH) and its decomposition product, benzo[1,2,3]thiodiazole-7-carboxylic acid (BTC), in grains by HPLC and LC/MS.
BTH and BTC were extracted from grains with 0.1 mol/L phosphate buffer (pH 8.0) and acetone. BTH was extracted from the acetone extract (adjusted to pH 7.5∼8.5) with diethylether-n-hexane (1 : 1). The remaining acetone extract was adjusted to <pH 3, and BTC was extracted with diethylether-n-hexane (1 : 1). The BTH extract was cleaned up on Florisil column after having been defatted by n-hexane-acetonitrile partition. The BTC extract was directly cleaned up on a Florisil column. BTH and BTC were determined by HPLC with UV or LC/MS (Scan or SIM). Recoveries of both compounds from rice, wheat and corn spiked at the level of 0.1 μg/g were in the range of 76.8∼86.7% (standard deviation was 0.3∼2.7%). The limits of detection were 0.003 μg/g (BTH) and 0.008 μg/g (BTC) by HPLC/UV.View full abstractDownload PDF (503K)
Reports
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Jin SUZUKI, Yasutaka KATSUKI, Hitoshi OGAWA, Keiko SUZUKI, Hiroko MATS ...2000 Volume 41 Issue 6 Pages 387-396
Published: December 25, 2000
Released on J-STAGE: January 11, 2008
JOURNAL FREE ACCESSTwenty-two trace elements (lithium, boron, aluminum, vanadium, chromium, manganese, iron, nickel, copper, zinc, gallium, arsenic, selenium, rubidium, strontium, molybdenum, cadmium, antimony, cesium, barium, lead and uranium) in 170 samples of bottled drinking water from Japanese market were determined by inductively coupled plasma mass spectrometry (ICP-MS). Sample solution of 100 mL of water spiked with 1 mL of nitric acid was subjected to ICP-MS. Recoveries of elements spiked in bottled drinking water were over 94%. Differences in concentration of elements depended on the source of the water. No sample contained more elements than the maximum levels recommended in the standards of manufacture for bottled drinking water. Four samples contained more elements than the maximum levels recommended in the Japanese water quality standard of drinking water.View full abstractDownload PDF (741K) -
Teruhiko HAGIWARA, Tetsuko YASUNO, Kazuo SAITO2000 Volume 41 Issue 6 Pages 397-400
Published: December 25, 2000
Released on J-STAGE: January 11, 2008
JOURNAL FREE ACCESSTotal bromine in 78 samples of natural food additive was determined. 2-Bromo-3-pentanone derived from the reaction of bromine with 3-pentanone was quantitated by capillary-gas chromatography with an electron capture detector (ECD-GC).
Total bromine higher than 1 ppm was detected in 23 samples out of 78 natural food additives. Among those samples, the concentrations of total bromine in licorice extract, licorice powder, coffee bean extract and stevia extract was 81 ppm, 25 ppm, 68 ppm and 21 ppm, respectively. In the manufacturing process of licorice extract from licorice powder, the total bromine concentration increased from 25 ppm to 91 ppm.
When sodium chloride was used as an additive in the preparation of natural food additive, bromide contained in the sodium chloride as an impurity was also detected.View full abstractDownload PDF (435K)
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