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Volume 48 (2007)
- Issue 6 Pages 163-
- Issue 5 Pages 125-
- Issue 4 Pages 83-
- Issue 3 Pages 41-
- Issue 2 Pages 27-
- Issue 1 Pages 1-
Volume 48, Issue 6
Displaying 1-6 of 6 articles from this issue
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Originals
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Aiko UJIIE, Hiroshi HASEBE, Yoshiko CHIBA, Noriaki YANAGITA2007 Volume 48 Issue 6 Pages 163-169
Published: December 25, 2007
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSA simultaneous determination method of saccharin (SA), sorbic acid (SOA), benzoic acid (BA), p-hydroxybenzoic acid ethyl (PHBA-Et), p-hydroxybenzoic acid isopropyl (PHBA-isoPr), p-hydroxybenzoic acid propyl (PHBA-Pr), p-hydroxybenzoic acid isobutyl (PHBA-isoBu) and p-hydroxybenzoic acid butyl (PHBA-Bu) in foods by HPLC was examined. A mixture of acetonitrile-water (1 : 1) was used to extract these additives from foods excluding liquid foods, while acetonitrile was used to extract them from liquid foods. HPLC was performed using a TSKgel ODS80Ts (4.6 mm i.d.×150 mm) column with a mobile phase of 0.01% formic acid solution containing 2 mmol/L-di-n-butyl (or amyl) ammonium acetate (A) and acetonitrile (B) under the following conditions: A/B=8 : 2 (0-8 min)→6 : 4 (15-32 min). Recoveries of these additives spiked in foods were 78-120%. The determination limits were 10 μg/g. As the identification method, examination by liquid chromatography with electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) was used. Unknown compounds were identified by detection of product ions from their precursor ions in the negative mode with multiple reaction monitoring, m/z 182>106 for SA, m/z 121>77 for BA, m/z 111> 67 for SOA and m/z 165>92 for PHBA-Et. Ratios of intensity of m/z 179>137 to m/z 179>92 were used for identification of isomers PHBA-isoPr and PHBA-Pr, and the ratios of intensity of m/z 193>137 to m/z 193>92 were used for isomers PHBA-isoBu and PHBA-Bu, because these isomers have very similar retention times on HPLC.View full abstractDownload PDF (661K) -
Takahiro WATANABE, Yuko SHIRAMASA, Satoshi FURUI, Kazumi KITTA, Yasuta ...2007 Volume 48 Issue 6 Pages 170-178
Published: December 25, 2007
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSWe developed a specific method to extract DNA from rice grain samples and modified the qualitative real-time PCR method provided by Bayer Co., Ltd. for reliable detection of the genetically modified (GM) rice variety, LLRice601, which has not undergone safety assessment for regulatory approval in Japan. Moreover, we conducted a data analysis to confirm the results obtained with real-time PCR. The yields of DNA extracted from powdered samples of rice grains were almost equal among 5 different varieties of rice, and there was no significant difference in the yield over three days. Reliable results were obtained using 50 ng of the extracted DNA as the template for real-time PCR. To examine the adequacy of the methods, we organized an inter-laboratory study with the participation of 2 laboratories, in which 80 test samples were analyzed in a blinded manner. The statistical analysis revealed no significant difference in the Ct value for the endogenous gene of the DNA samples and for the targeted DNA sequence of 0.1% samples. The limit of detection of the method was approximately 0.1%. Analysis of the fluorescence intensity of the PCR-amplified product of the construct-specific DNA sequence suggested that it may be reasonable to judge a sample as positive when a Ct value of less than 40 is obtained.View full abstractDownload PDF (898K)
Reports
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Atsuko TADA, Aino MASUDA, Naoki SUGIMOTO, Kazuo YAMAGATA, Takeshi YAMA ...2007 Volume 48 Issue 6 Pages 179-185
Published: December 25, 2007
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSThe differences in the constituents of ten ester-type gum bases used as natural food additives in Japan (urushi wax, carnauba wax, candelilla wax, rice bran wax, shellac wax, jojoba wax, bees wax, Japan wax, montan wax, and lanolin) were investigated. Several kinds of gum bases showed characteristic TLC patterns of lipids. In addition, compositions of fatty acid and alcohol moieties of esters in the gum bases were analyzed by GC/MS after methanolysis and hydrolysis, respectively. The results indicated that the varieties of fatty acids and alcohols and their compositions were characteristic for each gum base. These results will be useful for identification and discrimination of the ester-type gum bases.View full abstractDownload PDF (873K) -
Maki KOBAYASHI, Ichiro TAKANO, Yasuhiro TAMURA, Sanae TOMIZAWA, Yukina ...2007 Volume 48 Issue 6 Pages 186-193
Published: December 25, 2007
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSA survey of pesticide residues in 316 baby foods on the Tokyo market from April 1999 to June 2005 was carried out. Fifteen kinds of pesticides, including organophosphorus, organochlorine, carbamate, pyrethroid and others, were detected between at levels Tr (below 0.01 ppm) and 0.55 ppm from 28 samples.
The baby foods in which residual pesticides were detected were produced from flour, leaf vegetable and fruits. The pesticides were detected in baby foods produced with minimal processing, such as straining and squeezing.
For the baby foods containing pesticides, the amounts of intake of the pesticides were calculated from the recommended servings, and compared with the ADI values. Residual pesticide levels in baby food were between 0.06 and 16.6% of the ADI. Therefore, these foods should be safe in normal usage.View full abstractDownload PDF (592K) -
Masamitsu HIROTA, Chihiro SAWADA, Tsuyoshi SUZUKI, Chihiro UEDA, Mutsu ...2007 Volume 48 Issue 6 Pages 194-202
Published: December 25, 2007
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSWe tested for four kinds of allergic substances (egg, milk, wheat and peanuts) in 52 imported processed foods using immunochromatographic test kits (ITK). ELISA was also employed to confirm the effectiveness of the ITK. Among 92 data from 23 samples, allergic substances were detected in 9 cases with one kind of ITK, but not with the ELISA test. Among 116 data from 29 samples, 6 were negative with one kind of ITK and but positive with the other ITK. These results suggested that these 4 kinds of allergic substances in imported foods can be detected by using a double-check method with two kinds of ITK.View full abstractDownload PDF (527K) -
Hirotaka OBANA, Masakazu FURUTA, Yukio TANAKA2007 Volume 48 Issue 6 Pages 203-206
Published: December 25, 2007
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESS2-Alkylcyclobutanones, such as 2-dodecylcyclobutanone and 2-tetradecylcyclobutanone, were analyzed to assess the irradiation history of irradiated meats or fish, and cooked foods with irradiated ingredients, which had been stored frozen for up to one year. The purpose of the study was to show that irradiated meats could be detected even after having been stored in the distribution system. 2-Alkylcyclobutanones showed a small decrease in irradiated raw meats that had been stored frozen for one year. Cooked foods, such as pancake and fried chicken made with irradiated eggs and chicken, respectively, contained detectable levels of 2-alkylcyclobutanones after storage frozen for one year. The 2-alkylcyclobutanones became undetectable in highly dried samples, such as feed for lab animals, during the same period.View full abstractDownload PDF (230K)
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