The effects of hemolysis rate and hemolysis severity of porcine blood which was collected by the normal method from pigs upon the measured values of biochemical constituents of porcine serum were evaluated, and the following results were obtained.
1. Evaluation of hemolysis severity
Of 74 serum samples collected from pigs of the same number, 15 samples (20.3%) had no hemolysis (-), 21 (39.2%) showed (±) hemolysis, 12 (16.2%) (+) hemolysis, 15 (20.3%) (_??_) hemolysis and 3 (4.0%) showed (_??_) hemolysis. Thus, the samples with (+) or severer hemolysis accounted for approx. 40%.
2. Effects of hemolysis on measured values
1) Items in which neither overrating nor under-rating was caused by (±) hemolysis as compared with the non-hemolytic serum were TP (Total protein), serum protein fraction, BUN, ALP, GOT, GPT, LDH, LDH isozyme, Glu, T-cho, TG, PL, Fe, Ca, Mg and IP (inorganic phosphorus).
2) Item in which the measured values were significantly lower due to (+) or severer hemolysis as compared with that in non-hemolytic serum was LDH
1.
3) Items in which the measured values were significantly higher due to (_??_) or severer hemolysis as compared with that in non-hemolytic serum were LDH and LDH
5.
4) Items in which the measured values were significantly lower due to (_??_) hemolysis as compared with those in non-hemolytic serum were GOT, GPT, β-fraction and LDH
4.
5) Items in which the measured values were significantly lower due to (_??_) or severer hemolysis as compared with those in non-hemolytic serum were TG and PL.
6) Items in which neither overrating nor under-rating was caused by (_??_) hemolysis as compared with those of non-hemolytic serum were TP, serum protein fractions except β-fraction, A/G, BUN, ALP, LDH
2, LDH
3, Glu, T-cho, Fe, Ca, Mg and IP.
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